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2 edition of formation of ribosomes in tetrahymena pyriformis. found in the catalog.

formation of ribosomes in tetrahymena pyriformis.

Vagn Leick

formation of ribosomes in tetrahymena pyriformis.

by Vagn Leick

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  • 34 Currently reading

Published in Copenhagen .
Written in English

    Subjects:
  • Tetrahymena pyriformis.,
  • Ribosomes.

  • Classifications
    LC ClassificationsQL368.H87 L4
    The Physical Object
    Pagination108 p.
    Number of Pages108
    ID Numbers
    Open LibraryOL5470181M
    LC Control Number73173230

    The Distribution of Tetrahymena pyriformis This paper is a brief account of both amicronucleate and sexually active strains of Tetrahymena pyriformis and their distribution with some comments on their possible evolution. Citing Literature. Vol Issue 2. May Pages Related;Cited by:   The fraction of ribosomes loaded on polysomes is about 95% in logarithmically growing Tetrahymena thermophila, and about 4% in starved cells. Cytoplasmic extracts from cells in these two physiological states were used to develop column chromatographic methods for the purification of by:

    Leick V, Plesner P. Formation of ribosomes in Tetrahymena pyriformis. Biochim Biophys Acta. Dec 17; (2)– Gillespie D, Spiegelman S. A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane. J Mol Biol. Jul; 12 (3)– Suyama Y. Mitochondrial deoxyribonucleic acid of by:   We have examined the ribosomal structural proteins isolated from vegetatively growing Tetrahymena pyriformis and from cells that had been starved of all nutrients for 24 h. Reproducible, nonartifactual differences in protein complement, primarily associated with the large ribosomal subunit, were found.

    Summary. Temperature-sensitive mutants of Tetrahymena pyriformis which had previously been selected for their inability to grow at 38°C but which grew normally (or near normally) at 30°C were characterized with respect to their patterns of RNA and protein accumulation at both the permissive and nonpermissive temperatures. Out of such mutants, the majority (72) acted like wild type for Cited by: 4. The formation of several mitochondrial enzymes during the cell cycle in heat-synchronized Tetrahymena pyriformis ST. Exp Cell Res Cummings DJ. Mitochondrial genomes of the ciliates. Int Rev Cytol Curgy J-J, Ledoigt G, Stevens BJ, André J. Mitochondrial and cytoplasmic ribosomes from Tetrahymena pyriformis. J.


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Formation of ribosomes in tetrahymena pyriformis by Vagn Leick Download PDF EPUB FB2

The formation of ribosomal sub-units—derived from ribosomes by dialysis against low Mg 2+ concentration—has been studied by incorporation of RNA precursors into exponentially growing Tetrahymena cells. The S derived sub-units show a delayed kinetics of labeling as compared to the S derived by: 1.

Biochim Biophys Acta. Dec 17;(2) Formation of ribosomes in Tetrahymena pyriformis. Leick V, Plesner P. PMID: [PubMed - indexed for MEDLINE]Cited by: Formation of ribosomes in Tetrahymena pyriformis. Get this from a library. The formation of ribosomes in tetrahymena pyriformis.

[Vagn Leick]. Ribosome contents of growing and h-starved Tetrahymena pyriformis (strain B) were compared. These studies indicate that (a) starved cells contain 74% of the ribosomes found in growing cells, (b) growing cells devote 20% of their protein synthetic activity to ribosomal protein production, and (c) less than 3% of the protein synthesized in starved cells is ribosomal by: From the Whitman Laboratory, Department of Biology, University of Chicago, Chicago, Illinois.

Ribosome contents of growing and h-starved Tetrahymena pyriformis (strain B) were compared. These studies indicate that (a) starved cells contain 74% of the ribosomes found in growing cells, (b.

Some of the properties of the S ribosome of Tetrahymena pyriformis have been examined. We estimate from sedimentation coefficient (S) and intrinsic viscosity ( dl/g) a mol.

of 10 6 for the ribonucleoprotein particles. The RNA and protein ratio of the ribosomes is close to by:   The cellular site of synthesis of ribosomal RNA in Tetrahymena pyriformis was studied by analyzing the purified nuclear and cytoplasmic RNA from cells pulse labeled with uridine-(3)H.

The results of studies using zonal centrifugation in sucrose density gradients show that the ribosomal RNA is synthesized in the nucleus as a large precursor molecule sedimenting at 35S. Mitochondrial and cytoplasmic ribosomes from Tetrahymena pyriformis have been isolated and studied by the techniques of polyacrylamide gel electrophoresis and electron microscopy used in conjunction.

Although the two ribosome types show the same coefficient of sedimentation (80S) in sucrose gradients, they can be distinguished by gel electrophoresis: mitoribosomes migrate in a single Cited by: However, when Tetrahymena cells were transferred into a non‐nutrient medium, pronounced phosphorylation of a single ribosomal protein was observed.

During two‐dimensional polyacrylamide gel electrophoresis the phosphorylated ribosomal protein migrated in a manner virtually identical to that of the phosphorylated ribosomal protein S6 of rat Cited by: Biochemical data obtained from Tetrahymena pyriformis have suggested that this organism synthesizes ribosomes by the animal mode [15, 16].

However, a nucleolus in an expo- nentially growing Tetrahymena cell is able to synthesize a ribosomal particle at a rate approx. 10 times faster than a HeLa cell nucleolus [16].Cited by: The following problems concerning food vacuoles were studied by in vivo observations of Tetrahymena: (A) Formation of food vacuoles.

The process may be divided into 4 stages. Stage 1—gradual growth of the limiting membrane of the open food vacuole (of short duration). Stage 2—“filling up” of the fully expanded vacuole (of long duration).Cited by: 1.

Extracts from Tetrahymena lysosomes contained acid RNase and proteinase. At pH there was appreciable proteinase activity which was inhibited by a heat-stable protein present in cell sap. Lysosomal enzymes rapidly converted 80S ribosomes to subunits at pH Cited by: 5.

Proteose peptone (PP), an enzymatic digest of animal protein high in proteoses, is the traditional basis for most media used for growing Tetrahymenain the laboratory. In rich axenic media, PP is often supplemented by varying concentrations of yeast extract, glucose, and some form of by: Tetrahymena ribosomal DNA (rDNA) is an ideal system for studying eukaryotic DNA replication and gene transcription.

In this study, we developed a new method to isolate rDNA from Tetrahymena cells and used it to sequence and annotate the compl bp macronuclear rDNA minichromosome of Tetrahymena pyriformis, a species that lacks the germ-line micronucleus and is unable to undergo Author: Xiaojie Han, Xiaojie Han, Guanxiong Yan, Yang Ma, Wei Miao, Guangying Wang.

Effect of Actinomycin D and dl-p-Fluorophenylalanine on Ribosome Formation in Tetrahymena pyriformis. Nonidentity of ribosomal structural proteins in growing and starved Tetrahymena of ribosome catabolism in Tetrahymena pyriformis.

for the continuous formation of free ribosomes in resting. Abstract. The study of the Genetics of Tetrahymena pyriformis began about 20 years ago with the pioneering studies of Nanney and his co-workers.

It is now developing more rapidly and extensively than ever before. Allen and Gibson () have recently summarized the Genetics of T. pyriformisin “an attempt to reinterpret” it in relation to their own current views. A study on the effect of nalidixic acid on macromolecular metabolism, particularly of protein, in Tetrahymena pyriformis was performed.

It was shown that the compound is a potent inhibitor of. Mitochondrial and cytoplasmic ribosomes from Tetrahymena pyriformis have been isolated and studied by the techniques of polyacrylamide gel electrophoresis and electron microscopy used in .When C-palmitic acid is used to pulse label logarithmic cultures of Tetrahymena pyriformis, radioactivity appears in lipids of the various membrane types at vastly differing microsomes and postmicrosomal supernatant attain a high specific radioactivity within 1 min, while the membranes enveloping the cilia require several hours to reach the microsomal by: Summary.

Macronuclear envelopes were isolated from the ciliated protozoan Tetrahymena pyriformis GL, negatively stained and examined in the electron microscope.

The frequency of central granules in the macronuclear pores was evaluated in five different physiological states: (1) stationary phase of growth, (2) exponential phase of growth, (3) heat-synchronized cultures at the end of the heat Cited by: